Epigenetics Core Facility

Overview of Services

The Epigenetics Core Facility provides a variety of research resources and services that include:

At Bulk cell level:

- Nucleic acid extraction and fragmentation
- Protein-nucleic acid association (ChIP) from tissue and enriched population
- Quantitative gene expression analysis (qRT-PCR)
- In situ hybridization detection of transcriptional targets with spatial resolution (RNAscope and Basescope)
- Targeted DNA methylation analysis in specific genomic loci of interest (EpiTYPER),
- Next generation sequencing library preparation and Illumina sequencing (RNA-seq and ChIP-seq)

At Single Cell level:
- single cell transcriptome (10X Chromium 3' gene expression)
- single cell epigenome (10X Chromium ATAC-seq)
- single cell multigenome (10X Chromium Multiome)
- single cell gene expression (C1 and Biomark HD)

The facility also houses a sophisticated flow cytometry cell sorter that is equipped with 4 lasers and capable of detecting up to 11 colors. The sorter allows automated cytofluorimetric analysis and the sterile sorting of specific cell types, with biosafety protection.

Leadership

Jia Liu, Ph.D., Director

Phone: 212-413-3183

Email: jliu1@gc.cuny.edu

Location and hours of operation

Links and Resources

https://asrc.gc.cuny.edu/facilities/epigenetics/

Contacts